New: IncuCyte® NucLight Rapid Red Reagent for live-cell nuclear labeling

Last updated: 26th October, 2017

The IncuCyte Rapid Red Reagent for cell labeling is a cell permeable DNA stain that specifically stains nuclei in live cells and is ideally suited to the mix-and-read, real-time quantification of cell counting. Addition of the IncuCyte NucLight Rapid Red Reagent to normal healthy cells is non-perturbing to cell growth and morphology and provides homogenous staining of nuclei. When added to tissue culture medium, the inert stain crosses the cell membrane and has excellent specificity for DNA without the need for a wash step. With the IncuCyte® integrated analysis software, fluorescent objects can be quantified and background fluorescence minimized. This reagent can be multiplexed with IncuCyte® Annexin V, Caspase-3/7 or Cytotox reagents for simultaneous readouts of apoptosis or cytotoxicity alongside cell proliferation.

Key advantages:

  • Simple to use, mix-and-read reagent
  • Rapid, high efficiency cell labeling
  • Non-perturbing to cells at optimized dye concentrations
  • Ideal for homogenous labeling of multiple cell types

The IncuCyte NucLight Rapid Red Reagent has been validated for use with the IncuCyte® live-cell analysis system, using a simple mix-and-read protocol, enabling the real-time quantification of cell proliferation. At optimized concentrations, the reagent is non-perturbing and allows for long-term fluorescent measurements of cell nuclei and can be combined with our range of IncuCyte® Cell Health Reagents to quantify apoptosis or cytotoxicity alongside cell proliferation in a single well.

IncuCyte NucLight Rapid Red Reagent Quick Guide

nuclight protocol

NucLight Rapid Red Reagent is non-perturbing and enables high-efficiency labeling of cell nuclei

IncuCyte NucLight Rapid Red Figure 1

Optimized dye concentrations do not perturb cell health. NucLight Rapid Red Reagent labeled HT-1080 (adherent; left graph) and Jurkat (non-adherent; right graph) cells show equivalent cell growth profiles to non-labelled cells.

IncuCyte NucLight Rapid Red Figure 2

Rapid, high efficiency labelling of adherent and non-adherent cell types.  After addition of NucLight Rapid Red Reagent, all cells  in the population are labeled immediately and homogenously. Note the homogeneous labeling of cell nuclei and the healthy cell morphology. HT-1080 cells (left image) and Jurkats (right image) show representative nuclear labeling and red fluorescence. 

Multiplex with cell health reagents

IncuCyte NucLight Rapid Red Figure 3

Analysis of target tumor cell viability over 48 hours multiplexed with an apoptosis readout. HT-1080 cells were labeled with IncuCyte NucLight  Rapid Red Reagent and cultured in the presence of IncuCyte® Caspase-3/7 Green reagent. Cell death (apoptosis) is monitored in real time by measuring the loss of red object area (left) and increase of green object count (Caspase-3/7 signal) over time.

Visualize and quantify immune cell clustering and proliferation

IncuCyte NucLight Rapid Red Figure 4

Visualize changes in the phenotype of labeled immune cells. IncuCyte NucLight Rapid Red labeled human PBMC’s activated with anti-CD3 antibody and IL-2 induces T-cell aggregation or “clustering”. Images shown at are at 0, 60, and 96hrs. User friendly IncuCyte® software  enables accurate quantification of cell clustering over time.

IncuCyte® NucLight Rapid Red Reagent is supplied as a single vial (50 uL), sufficient for 100 to 200 tests in a 96-well plate.

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