Zika, Dengue & Chikungunya Virus Multiplex PCR Kit

Last updated: 25th October, 2016

Zika (ZIKV), Dengue (DENV) and Chikungunya (CHIKV) are arthropod-borne viruses (arboviruses) that share mosquitos of the Aedes genus as common vector, specifically Ae. aegypti and Ae. albopictus. Environmental changes, urbanization and the globalization of travel have enhanced the spread of ZIKV, DENV and CHIKV resulting in epidemics and the co-circulation in overlapping geographic areas as well as the possibility of viral co-infection within a single host. Currently the major endemic regions include Africa, Southeast Asia, the Pacific Islands and the Americas.

Besides, these arboviruses cause similar clinical presentations, especially in the initial stages of infection (fever, headache, skin rash, muscle and join pain, and gastrointestinal symptoms). In fact, due to neither virus possesses any specific distinguishing clinical features and the outcomes and management strategies for these three viruses are vastly different, an early and accurate diagnosis is imperative.

Therefore, ZIKV, DENV and CHIKV must be initially included in the differential diagnosis for a patient with suspicious clinical symptoms who is living in or returning from travel to an endemic area. Real Time RTPCR is a detection method commonly used during the acute phase of the infection. In order to do that, clinical specimens that can be tested including blood, serum, plasma, urine and others.

VIASURE Zika, Dengue & Chikungunya Real Time PCR Detection Kit is designed for the diagnosis of the Zika, Dengue and/or Chikungunya viruses in clinical samples. The detection is done in one step real time RT format where the reverse transcription and the subsequent amplification of specific target sequence occur in the same reaction well. The isolated RNA target is transcribed generating complementary DNA by reverse transcriptase which is followed by the amplification of a conserved region of the envelope gene (ZIKV), 3´Non coding region (DENV) and NSP1 gen (CHIKV), using specific primers and a fluorescent–labelled probe.

Key Benefits:

  • Ready-to-use kits, containing all the necessary components for the testing
  • Transport and storage at room temperature
  • Shelf life of 24 months for all qPCR products
  • Easy-to-use. It minimizes the number of manipulations reducing time and possible errors. Same protocol for all kits
  • High sensitivity, specificity and reproducibility
  • Positive and Negative controls included
  • Versatile. Possibility to analyse from 1 up to 96 samples per assay
  • Flexible and adaptable to a high number of commercial thermal cyclers

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