Meet the Muse® – Tiny but Mighty

Last updated: 12th October, 2020

Meet your Muse® – a robust, compact, and affordable instrument for automated cell analysis.

Assessment of cell concentration and viability are at the core of most cell biology experiments including investigating cell proliferation rates, optimising growth conditions, and normalizing cell data for further studies such as assessing the impacts of cytotoxic compounds.

Traditional manual techniques are extremely time consuming and require a high degree of technical competence to generate reproducible data. There are so many variables in play and it is not uncommon to see a high degree of variation among researchers with different experience levels.

Current automated methods rely on multiple platforms to provide these answers. This reduces flexibility, limits the ability to obtain comprehensive cell health information, and adds increased costs to researchers. Popular methods such as image-based analysis of Trypan blue-stained samples produce inconsistent results due to their dependence upon single-uptake dyes that do not effectively discriminate between the various states of cellular demise. Furthermore, in many cases extensive specialised training is required to operate these platforms efficiently (e.g. conventional Flow Cytometry).

Therefore, there is a crucial need for analytical tools that provide rapid, robust, and reproducible count and viability data to enable the efficient, daily execution of cellular research.

The Muse® Cell Analyzer is a unique instrument that enables multidimensional cell health analysis on a single platform.

It offers several advantages such as:

  • Simplified format enables researchers of varying experience levels to obtain a comprehensive picture of cellular health
  • Effortless acquisition and analysis of samples using mix-and-read assays
  • Highly simplified and intuitive touchscreen interface delivers rapid measurements of cell concentration, viability, apoptotic status, and cell cycle distribution
  • Highly sensitive, accurate and rapid detection using minimal cell numbers
  • Superior precision compared to traditional methods

Find out more about the Muse® Cell Analyzer

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What method do you currently use for fundamental cell counting, cell health, and signaling assays? See comparison and advantages of Muse® assays below:

 

Other Cell Analysis Techniques
Muse® Assays
Hemacytometer

  • Limited due to low-resolution dye uptake for viability determination
  • Subject to manual counting and calculation errors
Muse® Count and Viability Assay

  • Precise, fluorescence-based live/dead cell discrimination
  • Microcapillary fluidics for direct cell counts with industry-leading accuracy
Brightfield Microscopy-based Automated Hemocytometer/Cell Analyzer

  • Low accuracy based on small number of cells analyzed, and limited to count and viability measurement
Muse® Assays

  • Fluorescent reagents generate signal based on specificity for unique targets
  • Statistical significance achievable from rapid acquisition of thousands of cells
Western Blot and Other Bulk Assays

  • Population heterogeneity is lost when cells are pooled
  • Methods are time-consuming and require expertise
  • Reagents must be validated and optimized
  • Data is qualitative or semi-quantitative at best
Muse® Assays

  • Single-cell data is obtained from every sample
  • Extremely simple ‘pipette only’ assays
  • Validated and optimized for multiple cell types
Large, Open-system Flow Cytometer

  • Reagents must be validated for application
  • Optimization is time-consuming and uses precious sample
  • Software settings must be determined independently
Muse® Assays

  • Fluorescent reagents are chosen for compatibility with lasers and detectors and optimized on multiple cell types
  • Assays have validated, optimized sample preparation protocols and pre-set software settings

 


Simple analysis using mix-and-read assays:

Discover the range of Muse assays

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Discover Muse® Big 3 Assays. Click on the links below to find out more about what makes these cell health assays so popular:

Muse® Count and Viability Reagent Family

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Muse® Annexin V & Dead Cell Assay

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Muse® Cell Cycle Reagent Family

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By making cell health analysis simple, affordable and easily accessible, the Muse® Cell Analyzer can help integrate cell health analysis into everyday cell culture workflows.



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