The Glysite™ Explorer in situ PLA Glycan Detection Kit is a fully integrated tool for the spatial detection of glycans proximal to a protein of interest in FFPE tissues, FFPE cell pellets and fixed cells. This system integrates a curated panel of Vector’s Glysite Explorer lectins with Navinci’s proprietary in situ Proximity Ligation Assay (isPLA).
Researchers can flexibly select specific Glysite Explorer lectins covering the major categories of glycan types to pair with a primary antibody targeting their protein of interest. This provides a simple approach to understanding glycan-protein proximity with a spatial context.
The Glysite Explorer in situ PLA Glycan Detection Kit leverages Vector’s well-known immunohistochemistry portfolio and lectins with Navinci’s proven isPLA technology. This assay enables researchers to detect glycosylation proximal to specific proteins in a spatial context.
Simplified scheme of Glysite Explorer in situ PLA Glycan Detection Kit. A. Lectin binds to target glycan, and primary antibody binds to target protein. B. Oligo probes bind to primary antibody and lectin. C. When oligo probes are in proximity (~40nm), ligation occurs. D. Amplification and detection produces a colored precipitate at the site of proximity.
| Cat. No. | Product Name | Specificity |
|---|---|---|
| GEK-1399 | AAL Lectin (50x) | α-Fucose |
| GEK-1149 | ECL Lectin (50x) | Terminal type 2 LacNAc, Terminal type 2 LacdiNAc |
| GEK-1249 | GNL Lectin (50x) | Terminal α1-3 or α1-6 mannose |
| GEK-1159 | Jacalin Lectin (50x) | Core 1 and 3 O-glycans, 3-substituted GalNAcα |
| GEK-1049 | LCA Lectin (50x) | α1-6 fucose |
| GEK-1269 | MAL II Lectin (50x) | α2-3-sialylated Galβ1-3GalNAc in O-glycans, 3′ sulfated βGal |
| GEK-1119 | PHA-L Lectin (50x) | β1-6 branched N-glycans, Binds tri- and tetraantennary |
| GEK1309 | SNA Lectin (50x) | α2-6 Sialic acid |
| GEK-1359 | WFA Lectin (50x) | Terminal GalNAcβ, Terminal GalNAcα, Terminal multiantennary LacNAc |
| GEK-1029 | WGA Lectin (50x) | Terminal GlcNAcβ, Terminal GlcNAcα, Terminal NAc containing glycans |
