Gain your desired immunoassay sensitivity with the ultrasensitive FemtoQuest™ system
- Measure down to femtogram/mL levels
- Improved precision and robust data
- Perform dual-plex and single analyte analysis
- Low maintenance and automation compatible
- Use with a variety of off-the-shelf kits
- Integrated data analysis
- Lightweight fluidics-free benchtop instrument
- Cat. No. 95-0200-00
SMC® Technology
SMC technology provides maximum immunoassay performance while following a workflow similar to traditional ELISA technology, as shown below for dual-plex analysis. By combining a unique assay elution step and robust digital counting on the FemtoQuest system, SMC technology improves signal-to-noise ratios over traditional immunoassay technologies, enhancing quantification at both low and high levels of expression with one system. The FemtoQuest instrument can be used for measuring both single analyte and dual-plex assays.
Step 2-3
Antibodies translate each biomarker into a signal
Step 4
Fluorescent dye-labeled detection antibodies dissociate from the immunocomplex
Step 5
On the FemtoQuest instrument, signal is collected from free-floating fluorochromes using a rotating laser, camera, and counting individual molecules by an avalanche photodiode
Digital counting improves sensitivity and dynamic range
The FemtoQuest™ system captures all digital events counted above the background threshold. Using enhanced algorithms, digital events are computed across a spectrum of time series for a single standard. This digital counting of single molecules improves the assay sensitivity and extends the dynamic range beyond what can be achieved with traditional methods.
SMC® Singleplex High Sensitivity Kits
|
Product Description |
Cat. No. |
LLOQ (pg/mL)¹ |
Species² |
Sample Type³ |
|---|---|---|---|---|
|
Immunology/Inflammation |
||||
|
IFN-α2 |
03-0186-00 |
0.06 |
H |
P,S |
|
IFN-β1 |
03-0191-00 |
0.15 |
H |
P,S |
|
IFN-γ |
03-0181-00 |
0.03 |
H |
P,S |
|
IFN-λ1 |
03-0213-00 |
0.26 |
H |
P,S |
|
IL-1α |
03-0167-00 |
0.12 |
H |
P,S |
|
IL-1β |
03-0160-00 |
0.20 |
H |
P,S |
|
IL-2 |
03-0195-00 |
0.09 |
H |
P,S |
|
IL-4 |
03-0161-00 |
0.04 |
H |
P,S |
|
IL-5 |
03-0168-00 |
0.04 |
H |
P,S |
|
IL-6 |
03-0155-00 |
0.08 |
H |
P,S |
|
IL-10 |
03-0176-00 |
0.10 |
H |
P,S |
|
IL-12p70 |
03-0182-00 |
0.10 |
H |
P,S |
|
IL-13 |
03-0156-00 |
0.04 |
H |
P,S |
|
IL-17A/F Heterodimer |
03-0177-00 |
2.34 |
H |
P,S |
|
IL-17A |
03-0159-00 |
0.07 |
H |
P,S |
|
IL-17F |
03-0164-00 |
0.20 |
H |
P,S |
|
IL-18 |
03-0190-00 |
0.09 |
H |
P,S |
|
IL-22 |
03-0162-00 |
0.10 |
H |
P,S |
|
IL-22BP NEW |
03-0219-00 |
3.91 |
H |
P,S |
|
IL-23 |
03-0157-00 |
0.10 |
H |
P,S |
|
MCP-1 |
03-0187-00 |
0.15 |
H |
P,S |
|
TNFα |
03-0163-00 |
0.20 |
H |
P,S |
|
VEGF-A |
03-0180-00 |
0.20 |
H |
P,S |
|
Neuroscience |
||||
|
Phospho-α-Synuclein (Ser129) |
03-0188-00 |
0.10 |
H |
P,S,C |
|
Total α-Synuclein |
03-0196-00 |
1.95 |
H |
P,S,C |
|
Amyloid beta 1-40 |
03-0145-00 |
5.86 |
H |
P,C |
|
Amyloid beta 1-42 |
03-0146-00 |
0.98 |
H,M,R |
P,C |
|
BDNF |
03-0171-00 |
0.04 |
H |
P,S,C |
|
GFAP |
03-0203-00 |
0.23 |
H |
P,S,C |
|
NFL |
03-0220-00 |
0.87 |
H |
P,S,C |
|
NPTX2 |
03-0199-00 |
0.58 |
H |
P,S,C |
|
SNAP-25 |
03-0206-00 |
0.26 |
H |
P,S,C |
|
TDP-43 |
03-0205-00 |
5.21 |
H |
P,S,C |
|
Phospho-Tau181 |
03-0184-00 |
0.94 |
H |
P,S,C |
|
Phospho-Tau217 |
03-0210-00 |
1.95 |
H |
P,S,C |
|
Phospho-Tau231 |
03-0211-00 |
1.95 |
H |
P,S,C |
|
Total Tau |
03-0185-00 |
4.69 |
H |
P,S,C |
|
UCHL1 |
03-0183-00 |
2.60 |
H |
P,S,C |
|
Cancer |
||||
|
CTLA-4 NEW |
03-0220-00 |
0.30 |
H |
P,S |
|
PD-1 |
03-0207-00 |
3.47 |
H |
P,S |
|
PD-L1 |
03-0208-00 |
0.78 |
H |
P,S |
|
Cardiovascular |
||||
|
cTnI |
03-0154-00 |
0.69 |
H,Cy,R,C,GP |
P,S |
|
Metabolism/Endocrinology |
||||
|
Glucagon |
03-0153-00 |
0.78 |
H,M,R |
P |
|
Immunogenicity |
||||
|
IgE† |
03-0201-00 |
31.00 |
H |
P,S |
|
IgM† |
03-0204-00 |
205.00 |
H |
P,S |
|
Virology |
||||
|
SARS‑CoV‑2 RBD IgG* |
03-0193-00 |
NA* |
H |
P,S |
|
SARS‑CoV‑2 S1 IgA* |
03-0197-00 |
NA* |
H |
P,S |
New SMC® 2‑Plex High Sensitivity Kits for Use on the FemtoQuest™ Instrument
|
Product Description |
Cat. No. |
LLOQ (pg/mL)¹ |
Species² |
Sample Type³ |
|---|---|---|---|---|
|
Immunology/Inflammation |
||||
|
2‑Plex IL‑1β, IL‑10 NEW |
03-0218-2P |
0.04 (IL‑1β); 0.04 (IL‑10) |
H |
P,S |
|
2‑Plex TNFα, IL‑6 NEW |
03-0216-2P |
0.02 (TNFα); 0.06 (IL‑6) |
H |
P,S |
|
2‑Plex IL‑17A, IL‑17F NEW |
03-0214-2P |
0.02 (IL‑17A); 0.09 (IL‑17F) |
H |
P,S |
|
2‑Plex IL‑23, IL‑22 |
03-0215-2P |
0.04 (IL‑23); 0.09 (IL‑22) |
H |
P,S |
|
Cardiovascular |
||||
|
2‑Plex cTnI, IL‑6 NEW |
03-0217-2P |
0.35 (cTnI); 0.06 (IL‑6) |
H |
P,S |
¹ LLOQ: Lowest point on standard curve with CV ≤ 20% and accuracy within 20% of expected values. Lot‑specific LLOQ will be reported on the Certificate of Analysis.
² Optimized for first species type listed. Other listed species have been tested, but not optimized for peak performance. Species key: H=Human; M=Mouse; R=Rat; GP=Guinea Pig; Cy=Cynomolgus Monkey; C=Canine.
³ Optimized for use in sample type(s) listed. Key: P=Plasma; S=Serum; L=Lysate; U=Urine; C=Cerebrospinal Fluid (CSF). * Plate‑based assay. † Qualitative assay.
