Streamline your mRNA synthesis with TriLink’s CleanCap® IVT kits, which yield up to 1 mg per 100 µL reaction. Designed for efficiency and excellence, these kits include all components to generate high-quality mRNA using in vitro transcription (IVT) with CleanCap® co-transcriptional capping.
CleanCap® M6 IVT Kit, High Yield
- Includes Trilink’s newest cap analog, CleanCap® M6, for those aiming for even higher protein expression than with enzymatic caps and previous CleanCap® AG analogs
- May require 5’-UTR sequence review for optimal yield
| CleanCap® AG (3′ OMe) CleanScript™ IVT Kit | CleanCap® M6 IVT Kit, High Yield | |
|---|---|---|
| Cap analog | CleanCap® AG (3′ OMe) | CleanCap® M6 |
| Protein expression level | Higher than the original CleanCap® AG analog | Higher than enzymatic caps and previous CleanCap AG analogs |
| Construct to make | mRNA with AG start | mRNA with AG start |
| Considerations | Versatile | 5′ UTR sequence may affect yield |
| Included modified rNTP | N1-methylpseudouridine | N1-methylpseudouridine |
| Included 10X buffer | CleanScript™ IVT Buffer | M6 IVT Buffer |
| Included enzyme mix | AG CleanScribe™ RNA Polymerase Mix |
M6 CleanScribe™ RNA Polymerase Mix |
| Expected yield (per 100 μL rxn) |
0.8-1 mg | 0.8-1 mg* |
*Following its pulse-feed protocol
