TriLink – CleanCap AG (3′ OMe) CleanScript IVT Kit

TriLink’s CleanCap^® AG (3′ OMe) CleanScript™ IVT Kit provides essential components for mRNA synthesis using co-transcriptional capping by in vitro transcription (IVT). The kit components are sufficient to run 25 x 100 µL or 125 x 20 µL reactions reactions following our recommended IVT protocol with co-transcriptional capping. It includes a cap analog, modified and unmodified nucleotides, IVT enzymes, a reaction buffer, and a control DNA template. Each kit is expected to yield 20-25 mg of capped mRNA. The kit is designed for:

• Time and cost savings — Co-transcriptional capping protocol with all necessary reagents for a single-tube reaction providing high yield, fewer steps, and less handling
• High capping efficiency —Protocol with CleanCap AG (3′ OMe) cap analog to produce an optimal cap 1 with over 95% capping efficiency
• Increased mRNA yield — Buffer and kit components formulated using TriLink’s proprietary CleanScript IVT method for up to 10 mg/mL yield (or up to 1 mg mRNA from 5 µg linear plasmid) per 100 µL reaction
• Ultra-low double-stranded RNA (dsRNA) levels — up to 85% dsRNA reduction using CleanScribe RNA Polymerase (in place of wild-type T7 RNA polymerase) in combination with the CleanScript method (instead of standard IVT)
• Enhanced mRNA performance — Robust protein expression and low inflammatory responses from mRNA synthesized with CleanCap AG (3′ OMe), CleanScript method, CleanScribe RNA polymerase, and optional N1-methylpseudouridine

The kit includes:

• CleanCap^® Reagent AG (3′ OMe), 10 µmol
• Adenosine-5′-Triphosphate, 100 µmol
• Cytidine-5′-Triphosphate, 100 µmol
• Guanosine-5′-Triphosphate, 100 µmol
• Uridine-5′-Triphosphate, 100 µmol
• N1-Methyl-Pseudouridine-5′-Triphosphate, 100 µmol
• AG CleanScribe™ RNA Polymerase Mix, 250 µL
• 10X AG CleanScript™ IVT Buffer, 1 mL
• FLuc Control Plasmid, 25 µg

Product Details
Catalogue No	K-7413
Reaction Sizes	25 rxns (100 µL scale), 125 rxns (20 µL scale)
Recommended Storage	-15 to -25°C
Application	In vitro Transcription, co-transcriptional capping, mRNA synthesis
Cap	AG Start, Cap 1, CleanCap AG (3′ OMe)
Cap Analogs	CleanCap Cap Analogs

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TriLink mRNA workflow solutions TriLink BioTechnologies, part of Maravai LifeSciences, is a best-in-class contract development and manufacturing organisation (CDMO) for the synthesis of nucleic acids, NTPs and mRNA capping analogs. TriLink has scale-up expertise and unique mRNA, oligonucleotide & plasmid production capabilities for companies focused on therapeutic, vaccine, diagnostic and biopharmaceutical breakthroughs.

Cap Analogs	Nucleotides
CleanCap® cap analogs CleanCap M6 CleanCap AG (3ʹ OMe) CleanCap AG CleanCap AU	Modified and unmodified N1-methylpseudouridine Pseudouridine 5-methoxyuridine 5-methylcystidine rATP, rCTP, rGTP, rUTP
trilinkbiotech.com/ cleancap	trilinkbiotech.com/ nucleotides

Enzymes	Premade mRNAs
IVT enzymes CleanScribe™ RNA Polymerase Wild-type T7 RNA polymerase Engineered RNase inhibitor Inorganic pyrophosphatase	10 genes, 3 designs CleanCap M6 with N1-methylpsuedo U CleanCap AG with 5-methoxy U CleanCap AG with no base modification
trilinkbiotech.com/ ivtenzymes	trilinkbiotech.com/ premade-mrnas

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THESE PRODUCTS ARE NOT AVAILABLE FOR PURCHASE BY THE GENERAL PUBLIC.