VectaMount Mounting Medium Products
When applying a coverslip, selecting the appropriate mounting medium is essential to preserve the clarity, contrast, and stability of histochemical stains and precipitable enzyme substrates.
An optimal mounting medium for chromogenic substrates protects specimens from physical damage and prevents fading, diffusion, or loss of reaction products.
By effectively sealing and stabilizing the reaction precipitate, the correct mounting medium maintains precise localization and intensity of the chromogenic signals.
Vector Laboratories provides a portfolio of aqueous and non-aqueous mounting media specifically designed to meet the requirements of your experiment.
VectaMount Product Summary
| Product | Type | Catalog Number |
|---|---|---|
| VectaMount® PT Permanent Mounting Medium | Non-aqueous | H-5600-60 |
| VectaMount® Express Mounting Medium | Non-aqueous | H-5700-60 |
| VectaMount® AQ Aqueous Mounting Medium | Aqueous | H-5501-60 |
VectaMount PT Permanent Mounting Medium (H-5600)
A specially formulated, non-aqueous, optically clear solution for permanent preservation of histochemical stains or precipitable enzyme substrates in tissue sections or cell preparations.
Vector’s mounting medium has undergone extensive testing and validation with all Vector Laboratories substrates and counterstains so you can confidently preserve your samples.
Features:
- Permanent, Non-Aqueous Mounting: Provides stable, long-term preservation of specimens without the limitations associated with aqueous media.
- Color Preservation: VectaMount PT has been validated with Vector Laboratories’ substrates and counterstains, preserving the vibrant colors over time without loss of intensity or color shifts.
- Exceptional Optical Clarity: With an ideal refractive index (1.52), VectaMount PT ensures clear, artifact-free imaging.
- Reliable Long-Term Stability: Retains consistent adhesive strength, cohesive integrity, and color stability even during extended storage periods.
- Easy Application: Facilitates a uniform, thin mounting layer to enhance optical quality and simplify slide preparation.
- Versatile Usage: Effectively secures coverslips to both thin and thick specimens.Note: Highly flammable liquid and vapor. May be fatal if swallowed and enters airways. Causes skin irritation. Causes serious eye irritation.
VectaMount PT Permanent Mounting Medium (H-5600-60) is a different formulation than the discontinued VectaMount Permanent Mounting Medium (H-5000-60).
VectaMount Express Mounting Medium (H-5700)
VectaMount® Express is a non-aqueous clearing and mounting medium enabling the rapid mounting of cell and tissue specimens following immunohistochemistry (IHC) staining.
This novel formulation is engineered to enable mounting directly following staining, saving time by eliminating the need for extensive ethanol and clearing washes prior to coverslipping. Just stain your slides as per your usual workflow, briefly wash in isopropyl alcohol, and coverslip.
Features:
- Non-aqueous clearing and mounting medium for IHC-stained slides
- Eliminates the need for extensive ethanol washes and solvent-based clearing agents (e.g., xylene)
- Rapid drying formula for fast visualization of stained samples
- Preserves DAB staining for a minimum of 3 years and at least 18 months for other HRP and AP substrates at room temperature
- Compatible with horseradish peroxidase (HRP) and alkaline phosphatase (AP) enzyme substrates, as well as commonly used counterstains
- Optically clear formula with a refractive index of 1.49, resulting in high-quality imaging free of distortion or artifact
VectaMount AQ Aqueous Mounting Medium (H-5501)
VectaMount AQ Mounting Medium (H-5501) is a hardsetting mounting medium developed for use with enzymatic substrates, such as AEC, ImmPACT® AEC, and ImmPACT AMEC Red, whose reaction products are soluble in alcohol or other organic solvents.
In applications where aqueous mounting is preferred, VectaMount AQ is suitable for use with other substrates such as DAB, Vector® SG, BCIP/NBT, Vector Red, ImmPACT Vector Red, Vector Blue, ImmPACT DAB, ImmPACT DAB EqV, and ImmPACT SG.
Features:
- For aqueous mounting
- Compatible with many horseradish peroxidase and alkaline phosphatase substrates
