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Bethyl Laboratories – Custom Antibody Conjugation

Bethyl’s custom conjugation services deliver proven results, specialised dyes, and batch-to-batch consistency.

Bethyl specialises in conjugating antibodies to a wide range of labels including DyLight®, iFluor®, mFluor™, AZ dyes and other fluorophores, enzymes, and signal-amplifying conjugates, enabling consistency and confidence in every assay.

Partner with Bethyl’s proven expertise to cut time, conjugation variability, and accelerate your research from development to data.

Leverage Bethyl’s decades of conjugation experience and proven labeling technologies, without the stress. We deliver high-quality, consistent reagents that power your assays and accelerate discovery.

Bethyl’s custom conjugation solutions simplify your workflow and deliver consistent, high-quality results. From project initiation to final formatting, they provide end-to-end support covering antibody selection, conjugation, purification, and quality control.

01

Antibody Selection:
  • Choose from Bethyl’s extensive catalog of antibodies, and conjugation-ready carrier-free options or request custom antibody development.
  • For high-volume demands or longitudinal studies Bethyl offers Bulk antibody options to support scale and continuity

02

Conjugation Selection
  • Access the full range of labels, fluorophores, enzymes, and signal-amplifying conjugates.

03

Optional Quality Control:
  • Functional verification testing (e.g., flow cytometry, IF, IHC).

Every project includes post-conjugation 0.2 µm filtration, dye removal and F/P ratio for fluorophore conjugations, with optional functional testing and detailed data packages tailored to meet your specific needs.

Select the Ideal Conjugate for your Application

  Fluorescent Conjugates Ex / Em (nm) Similar Dyes (Rank Ordered)
UV (300 – 379 nm) DyLight® 350 353 / 432 AMCA, AF350
mFluor™ UV460 358 / 456 BUV496, Marina Blue®, Cascade Blue®, AMCA, AF350
VIOLET (380 – 439 nm) iFluor® 405 403 / 427 AF405, Pacific Blue®, BV421
mFluor™ Violet 450 406 / 445 V450, Pacific Blue®
mFluor™ Violet 500 410 / 501 V500, Pacific Green®, AmCyan, ATTO® 465
BLUE (440 – 509 nm) iFluor® 488 491 / 516 AF488, DL488, BB515
Cy®2 492 / 508 iF488, DL488, BB515, FITC
DyLight® 488 493 / 518 AF488, iF488, BB515, Cy2
AZDye 488 494 / 517 AF488, DL488, FITC, OG488
FITC 495 / 525 AF488, DL488, iF488, Cy2
RPE* 565 / 576 iF555, mF570, DL550, AF555
GREEN/YELLOW (510 – 599 nm) mFluor™ Blue 570 505 / 564 Cy3, AF555
Cy®3 554 / 568 AF555, iF555, DL550, PE/RPE
AZDye 555 555 / 572 Cy3, DL555, AF555
iFluor® 555 557 / 570 Cy3, AF555, DL550
DyLight® 550 562 / 576 Cy3, AF555, iF555
RPE* 565 / 576 SB Yellow 561, NF Y570, eF570
iFluor® 594 588 / 604 AF594, Texas-Red®, DL594
AZDye 594 590 / 617 AF594, CF594, DL594
RED (600 – 729 nm) DyLight® 594 593 / 618 iF594, AF594, Texas Red®
AZDye 647 649 / 666 Cy5, DL649
Cy®5 649 / 670 AF647, iF647, DL650, APC
DyLight® 650 652 / 677 AF647, iF647, Cy5, APC
iFluor® 647 656 / 670 AF647, Cy5, DL650
Cy®5.5 683 / 703 AF680, DL680, CFM680, IRDye 680LT, APC-Cy5.5
NIR / IR (730+ nm) mFluor™ Red 780 629 / 767 APC-AF750, APC-R700, iF750
DyLight® 755 754 / 776 iF750, AF750, Cy7
iFluor® 750 755 / 779 DL755, AF750, Cy7
DyLight® 680 692 / 712 AF680, Cy5.5, iF680, IRDye 680LT
DyLight® 800 777 / 790 IRDye 800, AF790



Explore Bethyl Laboratories extensive range of antibody conjugation

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THESE PRODUCTS ARE NOT AVAILABLE FOR PURCHASE BY THE GENERAL PUBLIC.

*These dyes have two maximum excitation wavelengths. Both can be used; optimal excitation will depend on application.
 
Abbreviations: Alexa Fluor® (AF), 7-Amino-4-Methylcoumarin-3-Acetic Acid (AMCA), Allophycocyanin (APC), Brilliant Blue (BB), Brilliant Violet (BV), BD Horizon™ BUV496 (BUV496), Cyanine (Cy®), DyLight® (DL), eFluor™ (eF), Fluorescein Isothiocyanate (FITC), Horseradish Peroxidase (HRP), iFluor® (iF), Infrared (IR), mFluor™ (mF), Near-Infrared (NIR), NovaFluor™ (NF), Oregon Green (OG), R-Phycoerythrin (RPE), StarBright™ (SB), Ultra-Violet (UV), BD Horizon™ V500 (V500).

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