Spotlight on LEF1 (EP310)

Last updated: 03rd February, 2022

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Chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) are small B-cell neoplasms that make up almost 1% of all diagnosed cancers.1 Included in the differential diagnosis of small B-cell lymphomas from SLL/ CLL are mantle cell lymphoma, follicular lymphoma, and marginal-zone lymphoma. Small B-cell lymphoma subtypes are morphologically similar and can be distinguished from each other by immunohistochemistry. Although the common immunophenotype for CLL/SLL is CD20+, cyclin D1-, SOX-11-, CD23+, CD5+ and CD10-, there are variable percentages for each antibody marker that will show aberrant expression.

CD23 is the standard diagnostic biomarker for CLL/SLL; however, 10-15% of CLL/SLL will be CD23-negative.2 Because of this phenomenon, other differential diagnostic markers exhibiting sensitivity and specificity for CLL/SLL are desirable. Lymphoid-enhancer-binding factor-1 (LEF1) is a nuclear marker that is expressed in up to 100% of CLL/SLL.3 One study cited LEF1 as having a 92% specificity and 70% sensitivity for CLL/SLL lesions.4 This diagnostic function of LEF1 justifies its inclusion into standard B-cell lymphoma panels

Benefits of Rabbit Monoclonal LEF1:

  • For in vitro diagnostic use
  • Nuclear visualization for easy interpretation
  • Differentiates CLL/SLL from mantle cell lymphoma, follicular lymphoma, and marginal-zone lymphoma
  • May detect CD23-negative CLL/SLL lesions


  1. http://seer.cancer.gov/statfacts/html/clyl.html
  2. http://surgpathcriteria.stanford.edu/bcell/sllcll/differentialdiagnosis.html
  3. Tandon B, et al. Mod Pathol. 2011; 24:1433-43.
  4. Menter T, et al. J Clin Pathol. 2015; 68:473-8.

Photo Information:

Right: LEF1 is expressed strongly and diffusely in small lymphocytic lymphoma.
Center: Mantle cell lymphoma tumor cells are negative for LEF1.
Left: LEF1 is negative in low-grade follicular lymphoma tumor cells.

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