Multiple Antigen Labeling with the ImmPRESS™ Polymer Detection System
The simplest system for use in a double labeling application is the ImmPRESS™ Polymer Detection System. ImmPRESS™ Polymer Reagents are available with either micropolymers of peroxidase (HRP) or alkaline phosphatase
(AP) detection enzymes attached to a variety of affinity-purified secondary antibodies. These one-step, non-biotin reagents are very sensitive with low non-specific background.
In addition, pairing ImmPRESS™ Polymer Reagents with Vector’s ImmPACT™ line of substrates produces sensitivities at least 2-4 times greater than with conventional substrates. Because the ImmPRESS™ Polymer System is a single step, the time for any multiple labeling experiment is shortened and the protocol simplified.
Vector ImmPRESS™ kits offer:
- One step detection after primary antibody.
- Avoids issues with endogenous biotin detection (non avidin/biotin detection system)
- Choice of enzyme system (peroxidase or alkaline phosphatase)
- Choice of species specific kit (such as anti-mouse, anti-rabbit, anti-goat IgG and universal)
- Straightforward single or multiple antigen detection on same tissue section
- Can be applied using either manual or automated staining applications
- Presented as pre-diluted, ready-to-use reagents i.e. no need to dilute a concentrated reagent
Recent Selected Published References that utilize ImmPRESS™ reagents
1) Mazur, A. et al (2016) PLoS ONE 11(3): e0150287
Describes single and double labeling using Vector ImmPRESS AP Anti-Rabbit IgG, ImmPACT Vector Red Substrate and Vector ImmPRESS Anti-Goat IgG Reagent, ImmPACT DAB Solution in mice breast tumor models.
2) Kohler, S. L. et al (2016) J Immunol. 196(6):2711-2722
Combined ISH and IHC using ImmPRESS anti-rabbit IgG and Vector NovaRED substrate, ImmPRESS AP anti-mouse IgG and Vector Red substrate in addition to ImmPRESS anti-mouse IgG and Vector SG substrate in human HIV-infected lymph node.
3) Pundavela, J. et al (2015) Molecular Oncology 9(8):1626-1635
Describes using Vector ImmPRESS anti-rabbit IgG, DAB and our Gill’s Hematoxylin (H-3401) in a human breast carcinoma study to identify the neuronal market PGP9.5.
4) Powell, E. et al (2016) Breast Cancer Research 18:13
Describes using Vector ImmPRESS anti-rabbit IgG, ImmPRESS anti-rat IgG and ImmPRESS M.O.M. kits to detect several antigens in human breast cancer xenografts grown in mice.
5) Zabini, D. et al (2015) Journal of Cellular and Molecular Medicine 19(5):1151-1161
Describes using Vector ImmPRESS anti-goat IgG and Vector VIP substrate, and ImmPRESS anti-rabbit IgG along with Vector NovaRED and DAB substrates in human hypertensive lung samples.